Ct值与起始模板的关系 每个模板的Ct值与该模板的起始拷贝数的对数存在线性关系公式如下 Ct-1lg1ExlgX0lgNlg1Ex n为扩增反应的循环次数X0为初始模板量Ex为扩增效率N为荧光扩增信号达到阈值强度时扩增产物的量.
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Ct值范围.
Ct 值的范围为 15-35 Ct 值小于 15 认为扩增在基线期范围内未达到荧光阈值 理想情况下 Ct 值与模板起始拷贝数的对数存在线性关系也就是标准曲线 通过标准曲线扩增效率为 100 时计算出基因单个拷贝数定量的 Ct 值在 35 左右若大于 35 理论上模板起始拷.
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